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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work B12 Shot Vitamin B12 1000 mcg – Nature's Bounty Buy More and Save:2 Box Deal $109.00 https://www.libertylabusa.com/cdn/shop/files/BPC-1575MG.jpg?v=1768819762&width=1946

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