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It is important to note that metformin can significantly affect the levels of various metabolites, including those involved in the glucose metabolism, lipid metabolism, tricarboxylic acid cycle (TCA), and urea cycle (t Hart et al., 2018a)
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PCR reaction conditions included 5 min degeneration (94 °C), 30 s degeneration (94 °C, 30 cycles), 30 s annealing (62.4 °C for 1800668 and 55 °C for rs1050450), 30 cycles of elongation for 1 min (72 °C), and a 10 min elongation (72 °C)
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