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Complex formation in vitro requires a direct interaction between CCSβ(Cu + )βCtr1 and either apoβ or ZnβboundβSOD1, or alternatively may occur from an interaction between preformed cytosolic CCSβZnβboundβSOD1 heterodimers and Cu + βCtr1
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These results indicate that valency contributes significantly to endocytosis efficiency, but cannot fully compensate for geometric constraints
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