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A differentiation protocol either produces hepatocyte-like cells or it does not, and this one has been repeated across laboratories and cell lines for a decade

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Figure 4 PPARγ Activity Involves in Altered DC Differentiation in MEHP-Treated FL-DCs As DEHP is rapidly metabolized to MEHP, a major metabolite in the serum (44), and MEHP is a partial agonist of PPARγ ( - populations, including late DC progenitors (CD11c + MHC class II - ), differentiating DCs (CD11c + MHC class II + ), and CD11c - subset (CD11c - MHC class II - and CD11c - MHC class II + ) ( Figure 4D )