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In contrast, many underutilized species, often referred to as orphan crops, receive limited research attention and funding despite their capacity to enhance nutrition, genetic diversity, and food security (Mukuwapasi et al., 2024) The FAO identifies amaranth as one such orphan crop highlighting its significant potential but also the absence of standardized agronomic protocols, phenotyping frameworks, genetic and molecular research, and improved varieties suitable for widespread cultivation (FAO, 2010)

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Using this retroviral transduction method, we were able to overproduce both HRASV12 and cyclin E1 in human osteosarcoma U2OS cells, as revealed by western blot analysis of total cell extracts (Supplementary Fig