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The supernatant was collected, and protein was quantified using the DC Rad Protein Assay kit

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Some common symptoms include irregular periods, abnormal body or facial hair growth, acne, weight gain, thinning hair, and infertility.23 Despite the condition’s name, you don’t need to have ovarian cysts to have PCOS.24 A diagnosis is made when you experience at least two of the three diagnostic criteria: irregular periods, high androgen levels, and multiple ovarian cysts that can be seen on an ultrasound