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PCR amplification was performed in triplicate by RT2 SYBR Green ROX (Qiagen, Hilden, Germany) by using StepOnePlus Real-Time PCR System (Applied Biosystems) at 95 °C for 10 min, 40 cycles at 95 °C for 15 s, all at 59 °C annealing temperature for 60 s followed by the melt curve
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US$40
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