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Antibiotic-treated mice were maintained in sterile caging with sterile food and water and handled aseptically for the remainder of the experiments

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FIGURE 6 Effects of ketogenic diet on hippocampal Sirt1/Nrf2 signaling pathway in chronic sleep deprivation exposed mice To evaluate the molecular mechanism by which KD exerted preventive effects on SD-induced AD, we examined the expression of Sirt1 and Nrf2 in the hippocampus by western blot (Figure 7A)

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