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These studies reveal that the relative abundance of N-terminal ubiquitin linkages is exceedingly low (to date several hundred sites have been identified), likely due to the fact that 80–90% of human proteins can be acetylated at their N-termini, a modification that would preclude N-terminal ubiquitylation ( Thus far the ubiquitin conjugating enzyme UBE2W is the only E2 known to catalyze ubiquitylation of the N-termini of proteins (Scaglione et al., 2013

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